ANTIMICROBIALS

 

 

Antimicrobials are widely used as preservatives to prevent microbial spoilage of preparations (due to micro-organisms being inadvertently introduced during their manufacture or use), as antiseptics to destroy or inhibit micro-organisms on living tissue (the intention is to limit or prevent the harmful effects of infection), and as disinfectants on inanimate objects and materials, the skin, and other membranes and in bodily cavities to destroy micro-organisms.  Some compounds are used for both preservation and disinfection, such as alcohol, quaternary ammonium compounds, thiomersal, and chlorocresol (1).

 

Preservatives are used in many products of concern to dermatology, such as topical pharmaceuticals (medications) and cosmetics and many household, industrial (where they are called biocides), and other products, like paper, napkins, and shoes (2).

 

Antimicrobials can cause irritant reactions, contact-allergic and photo-allergic reactions, as well as contact urticaria (3).

 

In addition to adverse skin reactions in patients who use topical preparations containing preservatives, antiseptic agents, or disinfectants, antimicrobials may also be responsible for occupational dermatitis in medical personnel and other professionals involved in the control or prevention of infection.  This article discusses the antimicrobial allergens we have encountered over the last 12 years i.e. 1985-1997.  Allergic reactions to antibiotics will not be covered here.

 

 

The allergens

 

Preservatives are the second most important sensitizing agents in cosmetics (e.g. 4).  They often consist of mixtures to enhance the antimicrobial spectrum.  In a recent study (5), we found that, although the methyl(chloro)isothiazolinone mixture ranks first, its frequency almost halved in the 1991-1996 period relative to the 1985-1990 period (4).  Indeed, this preservative was commonly used in the 1980s and was then a frequent cause of cosmetic contact allergy.  Since then, it has been generally replaced by preservatives that had already been on the market previously, such as formaldehyde (for which the frequency of reactions has almost doubled in recent years) and its releasers, such as quaternium-15, diazolidinylurea, imidazolidinylurea, hydantoine and bromo-nitropropanediol as well as a new preservative mixture containing methyldibromoglutaronitrile and phenoxyethanol.  This last preservative (also called Euxyl K400), which we have been routinely testing since April 1992, has now become an increasingly important allergen, the principal allergenic culprits being methyldibromoglutaronitrile or dibromodicyanobutane (6).  Of course, the spectrum of the different preservatives found to be allergens may differ from country to country (e.g. 7, 8).  Other cosmetic preservatives identified are chloroacetamide (a strong allergen even in minute concentrations), triclosan (mainly in deodorants), bradophen (a quaternary compound in one of the "udder creams" that are used to treat irritant hand dermatitis), captan (in hairdressing products), farnesol (in antiperspirants, cross-reacts with balsam of Peru), and bromonitrodioxane.

 


Contact sensitivity to preservatives such as parabens, which are often used in cosmetics may be primarily induced by topical pharmaceutical products, which are likely to facilitate the development of contact allergy because they are applied to eczematous or otherwise damaged skin.

 

The other preservatives identified in pharmaceutical products as allergens are thiomersal, for which most of the positive reactions encountered are irrelevant (e.g. 9), benzylalcohol (also used in perfumes), benzoic and sorbic acid, chlorocresol, benzalkonium chloride, and chlorbutol (both often used in eye drops).

 

The antimicrobials used as antiseptics are mainly thiomersal and other mercurials (e.g. merbromine), cetrimide, chloramine, nitrofurazone, quinoline derivatives (clioquinol, chlorquinaldolhol), chlorhexidine, alcohol, hexamidine, chloroxylenol, isopropanol, and sodium hypochlorite.  Some of these substances may also produce severe contact urticaria reactions, such as chloramine (e.g. 10) and chlorhexidine (e.g. 11).

 

One should not forget, however, that certain antiseptic medications (as well as disinfectants) may also sensitize through other ingredients present in the formulation; nonoxynols (45 positive reactions!) are non-ionic surfactants present in iodine-PVP solutions, and certain chlorhexidine and hexamidine preparations and are the typical examples in this regard (12).  In addition, lauramine oxide, an aliphatic amine present in a surgical scrub, caused 8 cases of professional hand dermatitis inmedical personnel.

 

Some of these antiseptics are also disinfectants, such as alcohol, isopropanol, quaternary ammoniumcompounds, chlorocresol, chloroxylenol, and formaldehyde.

Formaldehyde, together with glyoxal and particularly glutaraldehyde, causes occupational contact dermatitis (also airborne) in health care personnel (including dentists).  Dodicin or dodecyldi(aminoethyl)glycine is mainly used for surface disinfection and has been found to be the cause of allergic contact eczema in a swimming teacher.

 

The antimicrobial allergens found in industrial products (often called biocides) such as soluble oils, water-based paints, and glues are formaldehyde, isothiazolinone derivatives, benzotriazole, chloroacetamide, and methylolchloroacetamide.  TheBiobans were found to be specific causes of professional dermatitis due to metalworking fluids, while formaldehyde is used more often in household products.

 

Thiocyanomethylbenzothiazole is used to preserve leather.  Reactions to it were found in patients with shoe dermatitis.  In all of the cases except six, cross-sensitivity was found with other mercaptobenzothiazoles.  Thus it is difficult to determine whether it primarily sensitizes as a preservative agent or as a rubber additive in glues or rubber materials for shoes.

 

 


Conclusion

 

A wide variety of antimicrobials may be responsible for adverse skin reactions, including irritancy, allergic and photo-allergic contact eczema, and contact urticaria.  This article discusses allergic contact eczema due to their presence in topical pharmaceuticals, cosmetics, and household, industrial, and other products.  In some cases, they may cause occupational dermatitis, particularly in health-care workers.

 

 

References

 

1.      Martindale, The Extra Pharmacopoeia.  The Pharmaceutical Press, 30th edition, London, 1993, pp. 781 and1132.

2.      Rycroft RJG, Menne T, Frosch PJ (eds.).  Textbook of Contact Dermatitis, 2nd edition, Berlin, Springer-Verlag, 1995.

3.      de Groot AC, Weyland JW, Nater JP.  Unwanted effects of cosmetics and drugs used in dermatology.  Amsterdam, Elsevier, 1994.

4.      Dooms-Goossens A, Kerre S, Drieghe J, Bossuyt L, Degreef H.  Cosmetic products and their allegens.  Eur. J. Dermatol. 1992, 2:465-468.

5.      Goossens A, Merckx L.  L'allergie de contact aux cosmétiques.  In: Progrès en dermato-allergologie.  Marseille, M‚ discript, 1997, pp. 89-95.

6.      Jagtman B, van Geest T, van der Kley J.  Methyldibromoglutaronitrile is an important contact allergen in the Netherlands.  Contact Dermatitis 1996, 34:118-120.

7.      Perrenoud D, Birchner A, Hunziker T.  Frequency of sensitisation to 13 common preservatives in Switzerland.  Contact Dermatitis 1994, 30:276-279.

8.      Jacobs M-C, White IR, Rycroft RJG.  Patch testing with preservatives at St. John's from 1982-1993.  Contact Dermatitis 1995, 33:247-254.

9.      Müller H.  All these positive tests to thiomersal.  Contact Dermatitis 1994, 31:209-213.

10.  Dooms-Goossens A, Gevers B, Mertens A, Vanderheyden D.  Allergic contact urticaria due to chloramine.  Contact Dermatitis 1983, 9:319-320.

11.  Okano M, Nomura H, Hata S, Okada N, Sato K, Kitano Y, Tashiro H, Yoshimoto Y, Hama P.  Anaphylactic symptoms due to chlorhexidine gluconate.  Arch. Dermatol. 1989, 125:50-52.

12.  Dooms-Goossens A, Gidi de Alam A, Degreef H.  Contact sensitivity to nonoxynals: a cause of intolerance to antiseptic preparations.  In: Frosch A, Dooms-Goossens A, Lachapelle J-M, Rycroft RJG, Scheper RJ (eds.).  Current Topics in Contact Dermatitis.  Springer-Verlag, Berlin, 1983.

13.  Flyvholm M-A.  Identification of formaldehyde releasers and occurrence of formaldehyde and formaldehyde releasers in registered chemical products.  Am. J. of Ind. Med. 1993, 24:533-552.